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Safety Data Sheet (SDS) PE/Cy7 Anti-mouse CD71 Antibody     Product Data Sheet (PDF)    
PE/Cy7 Anti-mouse CD71 Antibody
1169055 25 µg $100.00       
1169060 100 µg $275.00       
Clone: RI7217
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: blocking of cellular proliferation.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Trowbridge I, et al. 1982. J. Cell. Physiol. 112:403.
2. Grisendi S, et al. 2005. Nature 437:147.
3. van Rooy I, et al. 2010. J. Control Release 150:30. PubMed
4. Willhelm BT, et al. 2011. Blood 117:27. PubMed
5. Okasi Y, et al. 2012. Exp Hematol. 40:143PubMed
6. Stojanov K, et al. 2012. Mol Pharm. 9:1620. PubMed

C57BL/6 splenocytes stained with RI7217

C57BL/6 splenocytes stained with RI7217 PE/Cy7 and TER-119 APC

C57BL/6 splenocytes stained with rat

C57BL/6 splenocytes stained with rat IgG2a PE/Cy7 isotype and TER-119 APC



Description:

CD71 is a 95 kD type II heterodimeric transmembrane glycoprotein that is also known as T9 and transferrin receptor. CD71 is expressed on proliferating cells, reticulocytes, and erythroid precursors. Its expression is very low on resting leukocytes. CD71 plays a role in the control of cellular proliferation by facilitating the uptake of iron via ferrotransferrin binding and the recycling of apotransferrin to the cell surface.

Other Names: Transferrin receptor, T9
Structure: Transferrin receptor and is 95 kD.
Distribution: Most dividing cells, the brain endothelium, and cycling hematopoietic progenitors.
Function: Mediates iron uptake.
Ligand Receptor: Transferrin.
Antigen References:

1. Hentze MW, et al. 1996. P. Natl. Acad. Sci. USA 93:8175.
2. Trowbridge IS, et al. 1993. Annu. Rev. Cell Biol. 9:129.
3. Trowbridge I, et al. 1982. J. Cell Physiol. 112:403.
4. Kuhn LC, et al. 1984. Cell 37:95.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
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