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Safety Data Sheet (SDS) Purified Anti-mouse CD53 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD53 Antibody
1223505 50 µg $65.00       
1223510 500 µg $245.00       
Clone: OX-79
Isotype: Rat IgM, κ
Reactivity: Mouse
Immunogen: BAB/14 mouse macrophage cell line RAW264
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
WB - Reported in the literature

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Puls KL, et al. 2002. Int. Immunol. 14(3):249.

C57BL/6 splenocytes stained with purified

C57BL/6 splenocytes stained with purified OX-79 conjugated to FITC and CD4 (GK1.5) PE

C57BL/6 splenocytes stained with rat

C57BL/6 splenocytes stained with rat IgM FITC isotype control and CD4 (GK1.5) PE



Description:

CD53 is a pan-leukocyte surface glycoprotein which spans the plasma membrane four times and is a member of the transmembrane 4 superfamily (TM4SF). CD53 is highly conserved in evolution, mouse CD53 was 91% identical to rat and 82% identical to human CD53. Mouse CD53 has a molecular mass of 35-45 kD and expresses on virtually all peripheral leukocytes, immature CD4-CD8- double negative thymocytes, and functionally mature CD4+CD8- or CD4-CD8+ single positive subsets. CD53 is proposed to play an important role in thymopoiesis and leukocyte signal transduction. It was reported that CD53 interact with a number of proteins, including CD2, CD9, CD19, and CD21. Cross-linking of CD53 promotes B cell activation. It was found that there is a strong correlation between CD53 expression and positive selection of thymocytes.

Other Names: OX-79
Structure: A 35-42 kD member of the tetraspanin/transmambrane-4 superfamily.
Distribution: Expressed on peripheral leukocytes, some immature CD4-CD8- double negative thymocytes, and functionally mature CD4+CD8- or CD4-CD8+ single positive subsets.
Function: Play an important role in leukocyte signal transduction, thymopoiesis, and positive selection of thymocytes.
Interaction: Interact with CD2, CD9, CD19, and CD21.
Antigen References:

1. Camo AM, et al. 1995. Eur. J. Immunol. 25(7):2090
2. Tomlinson MG, et al. 1995. Eur. J. Immunol. 25(8):2201
3. Angelisova P, et al. 1994. Immunogenetics. 39(4):249
4. Wright MD, et al. 1993. Int. Immunol. 5(2):209
5. Rasmussen A, et al. 1994. J. Immunol. 153:4997