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Safety Data Sheet (SDS) PE/Cy5 Anti-mouse CD127 IL-7Ralpha Antibody     Product Data Sheet (PDF)    
PE/Cy5 Anti-mouse CD127 (IL-7Rα) Antibody
1275075 50 µg $120.00       
1275080 200 µg $325.00       
Clone: A7R34
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: IL-7Ra-IgG1 fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

A7R34 is able to block clone SB/199 binding to IL-7R.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.15 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. Hashi H, et al. 2001. J. Immunol. 166:3702.
3. Taylor R, et al. 2007. J. Immunol. 178:5659.
4. Mazzon C, et al. 2011. Blood. 118:2733. PubMed
5. Jin J, et al. 2011. J. Immunol. doi:10.4049/jimmunol.1001238. PubMed

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with CD3 (145-2C11) FITC and A7R34 PE/Cy5 (top) or rat IgG2a,κ PE/Cy5 isotype control (bottom)





Description:

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.

Other Names: IL-7 receptor α chain, IL-7Rα
Structure: Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution: Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
Function: T cell and immature B cell proliferation and development
Ligand Receptor: IL-7
Antigen References:

1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. Okuno Y, et al. 2001. P. Natl. Acad. Sci. USA 99:6246.
3. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
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