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Safety Data Sheet (SDS) PE Anti-mouse/human CD276 B7-H3 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse/human CD276 (B7-H3) Antibody
1278025 25 µg $115.00       
Clone: MIH35
Isotype: Rat IgG2a, κ
Reactivity: Mouse, Cross-Reactivity: Human
Immunogen: Mouse B7-H3 transfected L cell and P815
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Hashiguchi M, et al. 2008. Proc Natl Acad Sci USA. 105(30):10495.
2. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed

Mouse B7-H3 transfected P815 cells

Mouse B7-H3 transfected P815 cells stained with MIH35 PE



Description:

B7-H3 is a type I transmembrane protein belonging to the B7 family of co-stimulatory proteins. B7-H3 is mostly expressed on professional APCs including B cells, macrophages, and dendritic cells at low levels. It is detected on various human and murine tumor cells, nasal and airway epithelial cells. Its expression on dendritic cells appears to be up-regulated by LPS. Initial studies have shown that B7-H3 provides a stimulatory signal to T cells. However, recent studies suggest a negative regulatory role for B7-H3 in T cell responses. Mouse B7-H3 protein inhibited T cell activation and effector cytokine production. Thus, the immunological function of B7-H3 remains unclear. B7-H3 is involved in the suppression of Th1-mediated immune responses and plays an important role in the development of pathogenic Th2 cells in a murine asthma model. Monoclonal antibody against B7-H3 enhances T cell proliferation in vitro and leads to exacerbated EAE in vivo. It has been reported that the Triggering Receptor Expressed on Myeloid cells (TREM)-like Transcript 2 (TLT-2, TREML2) is a receptor for B7-H3 in mice, although it remains controversial. Further studies are needed to identify the receptor of B7-H3.

Other Names: B7RP2
Structure: A 49.7 kD member of the B7 family of the Ig superfamily proteins.
Distribution: Mostly expressed on professional APCs, including B cells, macrophages, and dendritic cells at low level.
Function: Provides a stimulatory signal to T cells, plays a negative regulatory role in T cell responses as well.
Ligand Receptor: The receptor has not been completely identified, but it seems to be expressed on activated T cells.
Antigen References:

1. Nagashima O, et al. 2008. J. Immunol. 181:4062
2. Prasad DVR, et al. 2004. J. Immunol. 173:2500
3. Sun M, et al. 2002. J. Immunol. 168:6294
4. Xu J, et al. 2006. Cellular and Molecular Immunology. 3(3):235
5. Ford JW, et al. 2009. Curr Opin Immunol. 21(1):38
6. Leitner J, et al. 2009. Eur. J. Immunol. 2009. 39(7):1754