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Safety Data Sheet (SDS) FITC Anti-mouse TCR Valpha32 [b C] Antibody     Product Data Sheet (PDF)    
FITC Anti-mouse TCR Vα3.2 [b, C] Antibody
1277015 50 µg $85.00       
1277020 200 µg $195.00       
Clone: RR3-16
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: Mouse cytolytic T-cell clone OH6
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Utsunomiya Y, et al. 1991. Immunogenetics. 33(3):198
2. Utsunomiya Y, et al. 1989. J. Immunol. 143:2602

C57BL/6 mouse lymph node cells

C57BL/6 mouse lymph node cells stained with RR3-16 FITC and CD3 (145-2C11) APC

C57BL/6 lymph node cells stained

C57BL/6 lymph node cells stained with rat IgG2b FITC isotype control and CD3 (145-2C11) APC



Description:

TCR is a heterodimer composed of alpha and beta chains or gamma and delta chains. TCR Vα elements expression is largely independent of MHC haplotype. It is likely that the Vαs interact preferentially with conserved region of class I or class II molecules. RR3-16 antibody reacts with the Vα3.2 T-cell receptor (TCR) of mice having the b (e.g., C57BL/6) and c (e.g.,SJL, NOD) haplotypes, but not with the strains having the a ( Balb/c) or d (DBA/2) haplotype. Vα3.2 TCR is preferentially expressed by CD8+ T cells.

Other Names: Vκ 3.2[b,c] TCR [va3.2]
Distribution: subset of T cells.
Function: In combination of V β, Vκ3.2 recognizes MHC/peptide complex on the antigen-presenting cells.
Ligand Receptor: TCR recognizes MHC/peptide complex.
Antigen References:

1. Utsunomiya Y, et al. 1989. J. Immunol. 143:2602
2. Sim BC, et al. 1997. J. Immunol. 159(7):3322
3. Iwamoto A, et al. 1987. J. Exp. Med. 165(3):591