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Safety Data Sheet (SDS) PerCP/Cy55 Anti-mouse I-Ab Antibody     Product Data Sheet (PDF)    
PerCP/Cy5.5 Anti-mouse I-Ab Antibody
1182075 25 µg $115.00       
1182080 100 µg $290.00       
Clone: AF6-120.1
Isotype: Mouse (BALB/c) IgG2a, κ
Reactivity: Mouse b haplotype, Cross-reacts with H-2 k and H-2 u haplotypes. Does not react with other haplotypes (e.g., d, f, q, r, s).
Immunogen: C57BL/10J splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

 Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of frozen sections (acetone-fixed5; OCT-embedded, ethanol-fixed sections7), immunofluorescence microscopy3 (including acetone-fixed epidermal sheets6), immunoprecipitation7,8. Directly conjugated antibody was used for IF in (3) and (6) and for IHC in (5).

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application References:

1. Wall KA, et al. 1983. J. Immunol. 131:1056. (FC)
2. Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747. (FC)
3. Inaba K, et al. 1998. J. Exp. Med. 188:2163 (IF)
4. Hamrah P, et al. 2002. Invest Opthalmol Vis. Sci. 43:639 (IF)
5. Buono C, et al. 2003. Arterioscler. Thromb. Vasc. Biol. 23:454. (IHC)
6. Wang Z, et al. 2004. J. Immunol. 172:5924. (IHC IF)
7. Nakagawa TY, et al. 1999. Immunity 10:207. (IP)
8. Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC IP) PubMed
9. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
10. Weber GF, et al. 2014. J Exp Med 211:1243. PubMed

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with AF6-120.1 PerCP/Cy5.5



Description:

The AF6-120.1 antibody reacts with the I-Ab MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The AF6-120.1 antibody cross-reacts with H-2k and H-2u haplotypes; this antibody does not cross-react with other haplotypes (d, f, q, r, s).

Other Names: MHC class II
Structure: MHC class II
Distribution: B cell and activated T cells, APCs of H-2b mice
Function: Antigen presentation
Ligand Receptor: CD3/TCR, CD4
Antigen References:

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
COMMERCIAL USE shall include:

  • sale, lease, license or other transfer of the material or any material derived or produced from it;
  • sale, lease, license or other grant of rights to use this Material or any material derived or produced from it;
  • use of this material to perform services for a fee for third parties.

IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE RETURN THIS MATERIAL, UNOPENED TO Sony Biotechnology Inc., 2100 South Oak Street, Champaign, IL 61820, USA AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED.