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Safety Data Sheet (SDS) Purified Anti-mouse CD39 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD39 Antibody
1278510 100 µg $175.00       
Clone: 5F2
Isotype: Mouse IgG1, κ
Reactivity: Mouse
Immunogen: Purified recombinant mCD39-Ig, mCD39-expressing pcDNA3.1 plasmid, and B cells (CD39+)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
WB, ELISA - Reported in the literature

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Zhou Q, et al. 2009. Am J. Transplant. 9(10):2303

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained purified CD39 (clone 5F2, filled histogram) or mouse IgG1, κ isotype control, followed by anti-mouse IgG1 PE.



Description:

CD39, nucleoside triphosphate diphosphohydrolase-1 (NTPDase 1), is an ectoenzyme that degrades ATP to AMP. It is a member of ectonucleoside triphosphate dihydrolases (E-NTPDases) which are involved in regulation of extracellular nucleotide catabolism and controlling the extracellular nucleoside triphosphate pool (NTP). CD39 is the dominant member of this family in the immune system and involved in suppression of inflammation and control of platelet activation. CD39 is expressed on B cells, dendritic cells, and a subset of T cells including regulatory T cells and memory T cells. The coordinated expression of CD39/CD73 on Tregs and the adenosine A2A receptor on activated T effector cells generates immunosuppressive loops. In human studies, it has been reported that CD4+CD25-CD39+ T cells are T inducers.

Other Names: Entpd1, NTPDase1
Structure: A member of ectonucleoside triphosphate dihydrolases (E-NTPDases), an ectoenzyme that degrades ATP to AMP.
Distribution: CD39 is expressed on B cells, dendritic cells, and a subset of T cells
Function: CD39 is involved in suppression of inflammation and control of platelet activation. Coordinated expression of CD39/CD73 on Tregs and the adenosine A2A receptor on activated T effector cells generates immunosuppressive loops.
Antigen References:

1. Borsellino G et al. 2007. Blood 110(4):1225
2. Deaglio S et al. 2007. J. Exp. Med. 204(6):1257
3. Bynoe MS. et al. 2008. Trends Immunol. 29(3):99
4. Ndhlovu LC. et al. Eur. J. Immunol. 2009 Oct 28. [Epub ahead of print]