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Safety Data Sheet (SDS) Purified Anti-mouse CD88 C5aR Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD88 (C5aR) Antibody
1279010 100 µg $120.00       
Clone: 20/70
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: mC5aR transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested

Application Notes:

Additional reported applications for the relevant formats include: blocking of C5a binding.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Godau J, et al. 2004. J. Immunol. 173:3437.
2. Shushakova N, et al. 2002. J. Clin. Invest. 110:1823.
3. Corrales L, et al. 2012. J. Immunol. 189:4674. PubMed.

C57BL/6 bone marrow cells stained

C57BL/6 bone marrow cells stained with 20/70 PE and Ly-6G/Ly-6C (Gr-1) APC



Description:

CD88 is a single chain protein with seven membrane-spanning regions and a molecular weight of 43 kD. CD88 is expressed by neutrophils, eosinophils, and monocytes. There have been reports of CD88 expression in non-immune cells such as glial cells, cerebellar granule cells, cardiomyocytes, and vascular endothelial cells. CD88 is coupled to heterotrimeric G proteins such as Gi, and after C5a binding, the signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules, resulting in chemotaxis, granule enzyme release, and superoxide anion production.

Other Names: C5a ligand, C5a anaphylatoxin receptor, C5AR, C5R1, C5AR1
Structure: Single chain protein with seven membrane-spanning regions, 43 kD.
Distribution: Neutrophils, eosinophils, monocytes, glial cells, cerebellar granule cells, cardiomyocytes, and vascular endothelial cells.
Function: After C5a binding, the CD88 signal results in chemotaxis, granule enzyme release and superoxide anion production.
Interaction: CD88 is coupled to heterotrimeric G proteins such as Gi, The signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules.
Bioactivity/Activities: Chemotaxis, degranulation.
Antigen References:

1. Schraufstatter IU, et al. 2009. J. Immunol. 182:3827.
2. Ippel JH, et al. 2009. J. Biol. Chem. 284:12363.
3. Monk PN, et al. 2007. Br. J. Pharmacol. 152:429.
4. Griffin RS, et al. 2007. J. Neurosci. 27:8699.
5. Lee H, et al. 2006. Nat. Biotechnol. 24:1279.
6. Buck E and Wells JA, et al. 2005. P. Natl. Acad. Sci. USA 102:2719.