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Safety Data Sheet (SDS) PE Anti-mouse Galectin-9 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse Galectin-9 Antibody
1280515 50 µg $115.00       
1280520 200 µg $290.00       
Clone: RG9-35
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Recombinant mouse galectin-9
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Fukushima A, et al. 2008. Int. Arch. Allergy Immunol. 146:36. (FA)
2. Hou H, et al. 2014. PLoS One. 9:110585. PubMed

Intracellular staining of mouse thymocytes

Intracellular staining of mouse thymocytes with the RG9-35 PE



Description:

Galectin-9 is a mammalian lectin with a molecular weight of 40 kD that has two conserved carbohydrate recognition domains (CRDs) and forms homodimers. It recognizes N-acetyllactosamine (Galbeta1-4GlcNAc) and T-antigen (Galbeta1-3GalNAc). Tim-3 has been reported as its ligand. Galectin-9 is expressed by lymphocytes, dendritic cells, granulocytes, eosinophils, astrocytes, endothelial cells, fibroblasts, and thymus epithelial cells. It may be retained intracellularly or transported to the cell surface whereby cleavage generates a soluble form. Galectin-9 is involved in events such as cell aggregation, adhesion, chemotaxis, and apoptosis, and is important for the regulation of the immune response. Galectin-9 induces regulatory T cells, and suppresses Th1 and Th17 responses.

Other Names: Lgals9, Gal-9, Ecalectin
Structure: Homodimer, each chain is a 343 aa protein of 40 kD that has two conserved carbohydrate recognition domains (CRDs)
Distribution: Lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal/epithelial cells
Function: Cell aggregation, cell adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, induction of regulatory T cells
Ligand Receptor: Tim-3
Bioactivity/Activities: Chemotaxis, apoptosis, cell adhesion
Antigen References:

1. Klibi J, et al. 2009. Blood 113:1957
2. Seki M, et al. 2008. Clin Immunol 127:78
3. Tsuboi Y, et al. 2007. Clin Immunol 124:221
4. Zhu C, et al. 2005. Nat Immunol 6:1245
5. Dunphy JL, et al. 2002. J. Biol. Chem. 277:14916