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Safety Data Sheet (SDS) PerCP/Cy55 Anti-mouse H-2Kb Antibody     Product Data Sheet (PDF)    
PerCP/Cy5.5 Anti-mouse H-2Kb Antibody
1182575 25 µg $115.00       
1182580 100 µg $290.00       
Clone: AF6-88.5
Isotype: Mouse (BALB/c) IgG2a, κ
Reactivity: Mouse b haplotype, Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v)
Immunogen: C57BL mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunohistochemical staining of acetone-fixed frozen sections3. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application References:

1. Loken MR, et al. 1982. J. Immunol. Methods 50:R85.
2. Wall KA, et al. 1983. J. Immunol. 131:1056.
3. Andersson M, et al. 1998. J. Immunol. 161:6475.
4. Shao H, et al. 2005. J. Immunol. 175:1851.
5. Hui S, et al. 2005. J. Immunol. 175:1851. PubMed
6. Zhou K, et al. 2010. Cytotherapy. 12:735. PubMed
7. Desvignes L, et al. 2012. J Immunol. 188:6205. PubMed

C57BL/6 splenocytes stained with AF6-88.5

C57BL/6 splenocytes stained with AF6-88.5 PerCP/Cy5.5



Description:

The AF6-88.5 antibody reacts with H-2Kb MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kb haplotype. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).

Other Names: MHC class I
Structure: MHC class I
Distribution: Nucleated cells of the H-2Kb haplotype
Function: Antigen presentation
Ligand Receptor: CD3/TCR, CD8
Antigen References:

1. Watts C. 1997. Ann. Review Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Review Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
COMMERCIAL USE shall include:

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