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Safety Data Sheet (SDS) PE Anti-mouse TLT-2 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse TLT-2 Antibody
1283015 50 µg $115.00       
Clone: MIH47
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse TLT-2 Ig fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Hashiguchi M, et al. 2008. Proc. Nat. Acad. Sci. U. S. A. 105(30):10495

Mouse TLT-2 transfected cells stained

Mouse TLT-2 transfected cells stained with MIH47 PE

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with MIH47



Description:

Triggering receptor expressed on myeloid cell-like transcript 2 (TLT-2, TREML2) is a type I transmembrane protein in TREM family. It consists of a single extracellular Ig-like domain of the V-type, a transmembrane domain, and a short cytoplasmic domain. TLT-2 is expressed on myeloid , B cells , and macrophages. It was also found to express on CD8+ T cells and activated CD4+ T cells. It regulates innate immune response based on its expression profile, costimulates the activation of T cells. Transduction of TLT-2 into T cells resulted in enhanced IL-2 and IFN-gamma production. It was reported that B3-H7 is a receptor of TLT-2. Blockade of the B7-H3/ TLT-2 pathway with monoclonal antibodies against B7-H3 or TLT-2 efficiently inhibited hypersensitivity response. Further studies are needed to confirm the ligand.

Other Names: TREML2, TREM-like transcript 2,
Structure: A type I transmembrane protein in TREM family, consists of a single extracellular Ig-like domain of the V-type, a transmembrane domain, and a short cytoplasmic domain.
Distribution: Expressed on myeloid cells, B cells, macrophages, CD8+ T cells, and activated CD4+ T cells.
Function: Regulate innate immune response, IL-2, IFN-gamma production, and hypersensitivity response.
Antigen References:

1. King RG, et al. 2006. J. Immunol. 176:6012
2. Ford JW, et al. 2009. Curr Opin Immunol. 21(1):38
3. Leitner J, et al. 2009. Eur. J. Immunol. 39(7):1754