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Safety Data Sheet (SDS) PE Anti-mouse CD256 APRIL Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD256 (APRIL) Antibody
1283515 50 µg $100.00       
1283520 200 µg $290.00       
Clone: A3D8
Isotype: Armenian Hamster IgG
Reactivity: Mouse, Cross-Reactivity: Human
Immunogen: Fusion protein of murine Maltose binding protein (MBP) and soluble April
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Intracellular staining of the murine

Intracellular staining of the murine lymphoblastic cell line EL4 (black open histogram) and EL-4 cells transfected with mouse APRIL (orange filled) with A3D8 PE.

Intracellular staining of the murine

Intracellular staining of the murine macrophage like cell line P388D1 with A3D8 PE



Description:

CD256 (APRIL, TNFSF13, TALL2, TRDL1) a transmembranal protein of 26 kD, forms a homotrimeric molecule that is cleaved from the cell surface resulting in the soluble form of APRIL; it also forms heterotrimers with BLyS. Its receptor are TNFRSF13b/TACI and TNFRSF17/BCMA. Expressed by T cells, B cells, dendritic cells, monocytes, and macrophages, APRIL induces proliferation of T and B cells, promotes IgA class switching, downregulates T-cell dependent antibody responses, and regulates the growth of a variety of tumor cells.

Other Names: TNFSF13, TALL2, TRDL1
Structure: Transmembranal protein of 26 kD, forms a homotrimer that is cleaved from the cell surface resulting in the soluble form. forms heterotrimers with BLyS
Distribution: T cells, B cells, dendritic cells, monocytes, and macrophages
Function: Induces proliferation of T and B cells, promotes IgA class switching and downregulates T-cell dependent antibody responses, regulates the growth of a variety of tumor cells.
Ligand Receptor: TNFRSF13b/TACI, TNFRSF17/BCMA
Bioactivity/Activities: Proliferation, apoptosis inhibition, IgA class switch.
Antigen References:

1. Belnoue E, et al. 2008 Blood 111:2755.
2. Xie P, et al. 2007. Immunity 27:253.
3. Mohr E, et al. 2009. J. Immunol. 182:2113.
4. Tezuka H, et al. 2007. Nature 448:92.
5. Benson MJ, et al. 2008. J. Immunol. 180:3655.