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Safety Data Sheet (SDS) FITC Anti-mouse ESAM Antibody     Product Data Sheet (PDF)    
FITC Anti-mouse ESAM Antibody
1281025 50 µg $80.00       
Clone: 1G8/ESAM
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse bEND.3 endothelial cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Nasdala I, et al. 2002. J. Bio. Chem. 277:16294

Mouse endothelial cell line bEnd.3

Mouse endothelial cell line bEnd.3 stained with 1G8/ESAM FITC



Description:

Endothelial cell-selective adhesion molecule (ESAM) is a 55-kD membrane protein composed of two extracellular Ig domains, a single transmembrane domain, and a cytoplasmic domain. ESAM is predominantly expressed at endothelial junctions and on platelets, participating in the migration of neutrophils through the vessel wall by influencing endothelial cell contacts. It impacts vascular permeability and extravasation. Recently, it was reported that ESAM is a novel marker for murine hematopoietic stem cells (HSCs) in fetal liver. ESAM expression is correlated with HSC activity. The ESAMHi population was highly enriched for multipotent myeloid-erythroid progenitors and primitive progenitors with lymphpoietic activity, and exclusively reconstituted long-term lymphohematopoiesis in lethally irradiated recipients.

Other Names: Endothelial cell-selective adhesion molecule
Structure: Type I transmembraneA 55-kD membrane protein composed of two extracellular Ig domains, a single transmembrane domain, and a cytoplasmic domain protein complex
Distribution: Expressed at endothelial junctions, platelets, and murine hematopoietic stem cells.
Function: It impacts vascular permeability and extravasation. ESAM expression is involved in hematopoietic stem cell (HSC) activity.
Antigen References:

1. Wegmann F, et al. 2004. Exp Cell Res. 300:121
2. Ooi LAG, et al. 2008. Stem Cells. 27:653
3. Yokota T, et al. 2009. Blood 113:2871