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Safety Data Sheet (SDS) Purified Anti-mouse CD68 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD68 Antibody
1285005 50 µg $90.00       
1285010 500 µg $360.00       
Clone: FA-11
Isotype: Rat IgG2a
Reactivity: Mouse
Immunogen: Purified Con A receptor glycoproteins from the P815 cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

ICFC - Quality tested
FC - Validated
IP, WB, IHC - Reported in the literature

Application Notes:

Additional reported (for relevant formats) applications include: immunoprecipitation1, 2, Western Blot1, 2, and immunohistochemical staining of frozen sections2 and paraformaldehyde-fixed paraffin-embedded sections3.

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Silva RP, et al. 1999. Biochem. J. 338:687. (IP, WB)
2. Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP, WB, IHC)
3. Wu J, et al. 2008. P. Natl. Acad. Sci. USA 105:16934. (IHC)
4. Kayama H, et al. 2012. PNAS. 109:5010. PubMed
5. Park S, et al. 2013. Biomaterials. 34:598. PubMed
6. Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed
7. McKinstry SU, et al. 2014. J Neurosci. 34:9455. PubMed
8. Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed

Thioglycolate-elicited Balb/c peritoneal macrophages intracellularly

Thioglycolate-elicited Balb/c peritoneal macrophages intracellularly stained with FA-11 Alexa Fluor® 647



Description:

Mouse CD68, also known as macrosialin, is an 85-115 kD member of the lysosomal-associated membrane protein (LAMP) family. It is a heavily glycosylated and predominantly intracellular protein, mainly in late endosomes. Macrosialin is the murine homolog to the human macrophage glycoprotein CD68. It is expressed on tissue macrophages, Langerhans cells and at low levels on dendritic cells. Lamp proteins may have functions relating to cell-cell interaction or cell-ligand interaction. The biological function of CD68 is not completely understood.

Other Names: Macrosialin
Structure: A member of the lysosomal-associated membrane protein (lamp) family.
Distribution: Expressed on tissue macrophages, Langerhans cells, and at low levels on dendritic cells.
Function: Involved in cell-cell interaction or cell-ligand interaction, still not completely understood.
Antigen References:

1. Ramprasad MP, et al. 1996. Proc. Natl. Acad. Sci. USA 93:14833.
2. Smith MJ, et al. 1987. J. Cell. Sci. 87:113.