The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.
NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set and the Nuclear Factor Fixation and Permeabilization Buffer Set .
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB) 2. Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP) 3. Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF) 4. Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set (Cat# 424401). Cells were then stained with T-bet (clone 4B10) FITC (top) or mouse IgG1, κ FITC isotype control (bottom).
T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.
T-box expressed in T cells, T box 21, TBLYM
T-box transcription factor, approximately 58 kD.
Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
1. Szabo SJ, et al. 2000. Cell 100:655. 2. Szabo SJ, et al. 2002. Science 295:338. 3. Finotto S, et al. 2002. Science 295:336. 4. Mullen AC, et al. 2001. Science 292:1907.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Sony, the Sony logo, Sony Biotechnology Inc. and logo, iCyt, the iCyt logo EC800 and SY3200 are trademarks of Sony Corporation. Alexa Fluor® and Pacific Blue™ are registered trademarks of and licensed under patents assigned to Life Technologies Corporation. Cy and CyDye are trademarks of GE Healthcare Limited. iCyt products containing Cy5, Cy5.5 or Cy7 are manufactured under license from GE Healthcare under U.S. Patent Numbers 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. Brilliant Violet™, Brilliant Violet 421™, BV421™, Brilliant Violet 570™, BV570™, Brilliant Violet 605™, BV605™, Brilliant Violet 650™, and BV650™, Brilliant Violet 510™, BV510™, Brilliant Violet 711™, BV711™, Brilliant Violet 785™ and BV785™ are trademarks of Sirigen Group Ltd. PE-Dazzle 594™ is a trademark of Biolegend. All other trademarks are property of their respective owners. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.