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Safety Data Sheet (SDS) PE/Cy7 Anti-mouse CD117 C-kit Antibody     Product Data Sheet (PDF)    
PE/Cy7 Anti-mouse CD117 (c-kit) Antibody
1275555 25 µg $70.00       
1275560 100 µg $185.00       
Clone: ACK2
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: Murine IL-3 dependent mast cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Injection of ACK2 in mice of NOD genetic background is not recommended as mice develop immediate anaphylaxis, resulting in animal death. Treatment is possible with co-injection of Benadryl.3

ACK2 has been reported to block c-Kit function.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Yoshinaga K, et al. 1991. Development 113:689.
2. Broudy VC, et al. 1996. Blood 88:75.
3. Louvet C, et al. 2008. Proc. Natl. Acad. Sci. USA 105:18895.
4. Gong Y, et al. 2014. PLoS One. 9:95898. PuMed

Bone marrow cells from C57BL/6

Bone marrow cells from C57BL/6 mice stained with ACK2 PE/Cy7 and CD11b (M1/70) APC

Bone marrow cells from C57BL/6

Bone marrow cells from C57BL/6 mice stained with rat IgG2b PE/Cy7 isotype control and CD11b (M1/70) APC



Description:

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Other Names: c-KIT, Stem Cell Factor Receptor (SCFR)
Structure: Ig superfamily, 145 kD
Distribution: Hematopoietic stem cells, AML, mast cells, oocytes, interstitial theca cells, leydig cells
Function: Growth factor receptor, tyrosine kinase
Ligand Receptor: Stem Cell Factor (SCF)
Antigen References:

1. Barclay A et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ. et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P et al. 1986. Nature 320:415.
5. Witte ON. et al. 1990. Cell 63:5.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
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