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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse CD252 OX40L Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse CD252 (OX40L) Antibody
1144045 25 µg $90.00       
1144050 100 µg $190.00       
Clone: RM134L
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: Rat NRK-52E cell line transfected with mouse OX-40L.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The RM134L antibody can block the costimulatory activity of OX40L. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections3, and in vivo and in vitro blocking of OX-40L-OX-40 functional interaction1,2,4.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Application References:

1. Akiba H, et al. 1999. J. Immunol. 162:7058. (Block)
2. Akiba H, et al. 2000. J. Exp. Med. 191:375. (Block)
3. Hoshino A, et al. 2003. Eur. J. Immunol. 33:861. (IHC)
4. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Block)
5. Zheng X, et al. 2010. Invest Ophthalmol Vis Sci. 51:3076. PubMed

C57BL/6 splenocytes were stimulated with

C57BL/6 splenocytes were stimulated with anti-mouse IgM and CD40 antibodies for 3 days, then stained with OX40L (clone RM134L) Alexa Fluor® 647 (filled histogram) or rat IgG2b Alexa Fluor® 647 isotype control (open histogram).



Description:

CD252 is a 35 kD member of the TNF superfamily and is also known as OX40 ligand, OX40L, and CD134L. The CD252 is expressed on activated B cells and antigen presenting cells. CD252 interacts with OX40 antigen (CD134), expressed predominantly on activated T cells to increase proliferation and IL-2 production and to enhance proliferation and immunoglobulin secretion in activated B cells.

Other Names: OX-40L, CD134L, CD252, TNFSF4
Structure: NGF/TNF superfamily, 35 kD
Distribution: Activated B cells, antigen presenting cells
Function: B-cell/T-cell interaction, costimulation
Ligand Receptor: OX40(CD134)
Antigen References:

1. Akiba H, et al. 1999. J. Immunol. 162:7058.
2. Stüber E, et al. 1995. Immunity 2:507.
3. Baum PR, et al. 1994. EMBO J. 13:3992.


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.