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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-Bcl-2 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-Bcl-2 Antibody
3767545 25 µg $105.00       
3767550 100 µg $250.00       
Clone: BCL/10C4
Isotype: Mouse IgG1, κ
Reactivity: Mouse, Rat
Immunogen: N-terminal, Amino acid residues 61-76 of mouse Bcl-2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue4, immunocytochemical staining5, and immunoprecipitation5.

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our nuclear factor staining protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Application References:

1. Hsu YT, et al. 1997. J. Biol. Chem. 272:13829.
2. Zuber J, et al. 2011. Genes Dev. 25:1628. (WB) PubMed
3. Doi K, et al. 2012. Biochem Biophys Res Commun. 425:107. PubMed
4. Yamanaka, et al. 2003. J. Immunol. 170:816. (IHC)
5. Conus S, et al. 2000. EMBO J. 19:1534. (WB, IP, ICC)

C57BL/6 splenocytes intracellularly stained with

C57BL/6 splenocytes intracellularly stained with BCL/10C4 Alexa Fluor® 647.



Description:

Bcl-2 (B-cell leukemia 2) is an apoptotic protein and a member of the Bcl-2 family containing BH1-4 domains. Two reported isoforms exist α=25 kD; β=22 kD. The Bcl-2 protein forms homo- or hetero-dimers with other Bcl-2 family members. Bcl-2 is distributed in the outer mitochondrial membrane, the nuclear envelope, and the endoplasmic reticulum. This protein blocks apoptotic death by controlling mitochondrial membrane permeability. Cleavage of Bcl-2 can convert to pro-apoptotic (by cleavage of BH4 domain). Bcl-2 has been reported to regulate cell cycle progression via ROS. This protein is modified by ASK1/JNK1, PKC, ERKs, and stress-activated kinase phosphorylation and can be ubiquitinated. Bcl-2 has been shown to interact with Apaf-1, Raf-1, TP53BP2, caspase-3, and form heterodimers with Bax, Bad, Bak, Bcl-xL, and Bag-1. Clone BCL/10C4 has been shown to be useful for Western blotting, immunoprecipitation, and immunofluorescence of the mouse and rat Bcl-2 protein.

Other Names: B-cell leukemia 2
Structure: Apoptosis regulator proteins, Bcl-2 family, BH1-4 domains. Homo- or hetero-dimer; isoforms α, β 25 kD, 22 kD
Distribution: Outer mitochondrial membrane, intracellular membrane nuclear envelope, endoplasmic reticulum
Function: Blocks apoptotic death by controlling the mitochondrial membrane permeability. Converted to pro-apoptotic activity by cleavage of BH4 domain. Regulates cell cycle progression via ROS
Regulation: Phosphorylation by ASK1/JNK1, PKC, ERKs, stress-activated kinases
Modification: Phosphorylation, Ubiquitination
Antigen References:

1. Tsujimoto Y, et al. 1986 P. Natl. Acad. Sci. USA 83:5214.
2. Yang E, et al. 1995. Cell 80:285.
3. Huang Z, et al. 2000. Oncogene 19:6627.
4. Deng X, et al. 2003. Blood. 102:3179.


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