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Safety Data Sheet (SDS) Purified Anti-mouse Ly49G Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse Ly49G Antibody
1287010 500 µg $210.00       
Clone: AT8
Isotype: Mouse IgG2a, κ
Reactivity: Mouse, Cross-Reactivity: No
Immunogen: IL-2-activated 129/J NK cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:
  1. Makrigiannis AP, et al. 2004.  J. Immunol.  172:1414.
Balb/c mouse splenocytes stained with

Balb/c mouse splenocytes stained with CD49b (DX5) APC and AT8 PE (top) or mouse IgG2a, κ PE isotype control (bottom).





Description:

Ly49G is a type II transmembrane protein belonging to the family of Ly49 proteins, each having specificity for a small repertoire of MHC I alleles that are generally recognized in a peptide-independent manner.   The Ly49 family of MHC class I receptors shows a high degree of variability between mouse strains.  It is expressed on a subset of NK cells and some NKT cell lines.  The AT8 antibody reacts with the Ly49G receptor from BALB/c, 129/J, 129Sv/J, SJL, FVB/N, A/J, C3H/HeJ, C3H/HeNCr, CBA/J, and CBA/NCr mice.

Other Names: Ly-49G, Ly49g, Killer cell lectin-like receptor 7, Klra7
Structure: A type II transmembrane protein belonging to Ly49 family.
Distribution: Subset of NK cells and some NKT cell lines
Function: Allele-specific MHC recognition
Ligand Receptor: MHC class I molecules
Antigen References:
  1. Lavender KJ, et al. 2006. J. Immunol. 177:8578.
  2. Pascal V, et al. 2007. J. Immunol.. 179:1751.
  3. Lavender KJ, et al. 2007. J. Immunol. 179:6856.
  4. Rouhi A, et al. 2007. Mol. Immunol. 44(5):821.