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Safety Data Sheet (SDS) APC Anti-mouse ESAM Antibody     Product Data Sheet (PDF)    
APC Anti-mouse ESAM Antibody
1281035 25 µg $100.00       
Clone: 1G8/ESAM
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse bEND.3 endothelial cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported (for relevant formats) applications include: immunoprecipitation, immunohistochemical staining of frozen sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Nasdala I, et al. 2002. J. Bio. Chem. 277:16294.

Mouse endothelial cell line bEnd.3

Mouse endothelial cell line bEnd.3 stained with 1G8/ESAM APC



Description:

Endothelial cell-selective adhesion molecule (ESAM) is a 55-kD membrane protein composed of two extracellular Ig domains, a single transmembrane domain, and a cytoplasmic domain. ESAM is predominantly expressed at endothelial junctions and on platelets, participating in the migration of neutrophils through the vessel wall by influencing endothelial cell contacts. It impacts vascular permeability and extravasation process. Recently, it was reported that ESAM is a novel marker for murine hematopoietic stem cells (HSCs) in fetal liver. ESAM expression is correlated with HSC activity. The ESAMHi population was highly enriched for multipotent myeloid-erythroid progenitors and primitive progenitors with lymphpoietic activity, and exclusively reconstituted long-term lymphohematopoiesis in lethally irradiated recipients.

Other Names: Endothelial cell-selective adhesion molecule
Structure: A 55-kD membrane protein composed of two extracellular Ig domains, a single transmembrane domain, and a cytoplasmic domain.
Distribution: Expressed at endothelial junctions, platelets, and murine hematopoietic stem cells.
Function: It impacts vascular permeability and extravasation process. ESAM expression is involved in hematopoietic stem cell (HSC) activity.
Antigen References:

1. Wegmann F, et al. 2004. Exp Cell Res. 300:121.
2. Ooi LAG, et al. 2008. Stem Cells 27:653.
3. Yokota T, et al. 2009. Blood 113(13):2871.