The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Dent AL, et al. 1990. Nature 343:714. 2. Kelly KA, et al. 1993. Int. Immunol. 5:331. 3. Sperling AI, et al. 1992. J. Immunol. 149:3200. 4. Sperling AI, et al. 1997. J. Immunol. 159:86.
C57BL/6 splenocytes stained with UC3-10A6 FITC and CD3 APC
T cell receptor (TCR) Vγ2 bearing T lymphocytes make up a significant proportion of γδ TCR cells in late fetal and adult peripheral lymphoid tissues. TCR γδ T cells may play a role in immunological surveillance for stress-induced self-antigens. The frequency of Vγ2 expression in different strains varied from 12% to 54% in the TCR γδ repertoire. Variations in the levels of Vγ2+ cells are not associated with MHC haplotype. High Vγ2 expression is influenced by the TCR-δ locus. Expanding Vγ2+ TCRγδ cells in B6 mice overwhelmingly use a Vδ7+ δ chain except in the DBA/2 strain.
Vγ2 T-cell receptor, T cell receptor gamma 2
A member of Ig superfamily, associated with TCR δ chain and CD3 complex.
T cell subset in fetal and adult peripheral lymphoid tissues and thymus.
Might play a role in a previously unrecognized form of immunological surveillance for stress-induced self-antigens.
1. Allison JP, et al. 1991. Annu. Rev. Immunol. 9:679. 2. O’Brien RL, et al. 2000. J. Immunol. 165:6472.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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