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Safety Data Sheet (SDS) Purified Anti-mouse CD26 DPP-4 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD26 (DPP-4) Antibody
1289005 50 µg $80.00       
1289010 500 µg $340.00       
Clone: H194-112
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: BALB/c thymocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IP, IHC - Reported in the literature

Application Notes:

Additional reported applications include: in vitro activation of thymocytes1, immunoprecipitation from cell lysates and cell-free supernatants1,2 and immunohistochemical staining of frozen tissue sections1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Naquet P, et al. 1988. J. Immunol. 141:4101.
2. Vivier I, et al. 1991. J. Immunol. 147:447.
3. Sen A, et al. 2012. PNAS PubMed
3. Coleman CM, et al. 2014. J Gen Virol. 95:408. PubMed

 C57BL/6 splenocytes stained with

C57BL/6 splenocytes stained with purified H194-112 conjugated with PE and CD3 (17A2) Alexa Fluor® 647



Description:

CD26, also known as DPP IV or THAM, is a 220 kD type II transmembrane homodimer. It consists of an α/β hydrolase domain and an eight-blade beta-propeller domain.  After proteolysis of the membrane-bound CD26, a soluble form of DPP IV is released. CD26 is expressed on thymocytes (development dependent), T cells, B cells, NK cells, and macrophages. It is involved in T cell costimulation, endothelial cell migration and proteolysis processes.

Other Names: Dipeptidyl peptidase 4, THAM
Structure: Homodimer, each monomer is a single chain type II transmembranal molecule of 110 kD that consists of two domains; soluble form is released after proteolysis of the membrane form
Distribution: Mature thymocytes, activated T cells, B cells, NK cells, macrophages
Function: T cell costimulation, endothelial cell migration, proteolysis
Ligand Receptor: ADA, collagen
Interaction: CAV1, IGF2R, PTPRC, BCL10, CARD1, IKBKB
Antigen References:

1. Cooper KG, et al. 2009. Infect. Immun. 77:2447.
2. Eltzschig HK, et al. 2006. Blood 108:1602.
3. Peranteau WH, et al. 2006. Blood 108:4268.