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Safety Data Sheet (SDS) Purified Anti-mouse CD144 VE-cadherin Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD144 (VE-cadherin) Antibody
1290505 50 µg $80.00       
Clone: VECD1
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: VE-cadherin protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IP, WB, IF - Reported in the literature

Application Notes:

Additional reported applications (for relevant formats) include: Immunoprecipitation1, Western Blot1, functional assay2, 3 by inhibiting vascular endothelial cell-cell interactions and immunocytochemistry staining of cultured cell lines4.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Allport JR, et al. 2002. J. Leukoc. Biol. 71:821. (IP WB)
2. Hirashima M, et al. 2009. Blood 93:1253. (Block)
3. Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362. (Block)
4. Matsumura K, et al. 2003. Blood 101:1367. (IF)
5. Hirashima M, et al. 2009. Blood 101:2261. (FC)
6. Urbschat A, et al. 2014. PLoS One. 9:84432. PubMed

Mouse endothelial cell b.End.3 stained

Mouse endothelial cell b.End.3 stained with purified VECD1 conjugated with PE



Description:

CD144, also known as vascular endothelial-cadherin (VE-cadherin), is a 120 kD member of the type II Cadherin family.  It is an endothelial specific hemophilic adhesion molecule involved in endothelial cell survival, migration, contact-dependent growth inhibition and homophilic adhesion.  VE-cadherin is essential for maintaining the integrity of the endothelial barrier in vivo.

Other Names: Vascular endothelial-cadherin, VE-cadherin
Structure: A 120 kD member of type II Cadherin family
Distribution: Endothelial cells
Function: Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion.
Antigen References:

1. Gotsch U, et al. 1997. J. Cell Sci. 110:583.
2. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729.