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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse CD144 VE-cadherin Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse CD144 (VE-cadherin) Antibody
1290025 25 µg $150.00       
1290030 100 µg $345.00       
Clone: BV13
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: VE-cadherin-Ig fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Clone BV13 recognizes an epitope between aa 45 and 56, and has a binding affinity of 5-15 nM.5 Additional reported applications (for relevant formats) include: Western blotting1, blocking of cell interactions in vivo1, and immunofluorescence microscopy4.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Application References:

1. Corada M, et al. 1999. P. Natl. Acad. Sci. USA 96:9815. (WB, Block)
2. Liao F, et al. 2000. Cancer Res. 60:6805. (FC)
3. Crosby CV, et al. 2005. Blood 105:2771. (FC)
4. Liao F, et al. 2002. Cancer Res. 62:2567. (IF)
5. May C, et al. 2005. Blood 105:4337. (epitope)

Mouse endothelial cells b.End.3 stained

Mouse endothelial cells b.End.3 stained with BV13 Alexa Fluor® 647



Description:

CD144, also known as vascular endothelial-cadherin (VE-cadherin), is a 120 kD member of the type II Cadherin family.  It is an endothelial specific hemophilic adhesion molecule involved in endothelial cell survival, migration, contact-dependent growth inhibition, and homophilic adhesion.  VE-cadherin is essential for maintaining the integrity of the endothelial barrier in vivo.

Other Names: Vascular endothelial-cadherin
Structure: A 120 kD member of type II Cadherin family
Distribution: Endothelial cells
Function: Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion
Ligand Receptor: CD144
Antigen References:

1. Allport JR, et al. 2002. J. Leukocyte Biol. 71:821.
2. Hirashima M, et al. 2009. Blood 93:1253.
3. Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362.
4. Matsumura K, et al. 2003. Blood 101:1367.
5. Hirashima M, et al. 2009. Blood 101:2261.
6. Gotsch U, et al. 1997. J. Cell Sci. 110:583.
7. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729.


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