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Safety Data Sheet (SDS) APC Anti-mouse/rat CD126 IL-6Ralpha Chain Antibody     Product Data Sheet (PDF)    
APC Anti-mouse/rat CD126 (IL-6Rα Chain) Antibody
1179055 25 µg $100.00       
1179060 100 µg $245.00       
Clone: D7715A7
Isotype: Rat IgG2b, κ
Reactivity: Mouse, Rat
Immunogen: OKT4 hybridoma cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2,5in vivo receptor blocking1-2,4-6,8, and in vitro neutralization7. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody or biotinylated anti-rat IgG second step , followed by SAv-PE ).

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Coulie PG, et al. 1990. Curr. Top. Microbiol. Immunol. 166:43. (Block, IP)
2. Vink A, et al. 1990. J. Exp. Med. 172:997. (Block, IP)
3. Sun R, et al. 2004. J. Immunol. 172:5648. (FC)
4. McKinney WJ, et al. 1998. Am. J. Respir. Cell Mol. Biol. 18:696. (Block)
5. Weigert C, et al. 2006. J. Biol. Chem. 281:7060. (Block, IP)
6. Koizumi N, et al. 2007. J. Immunol. 178:1767. (Block)
7. Walker F, et al. 2008. Blood 111:3978. (Neut) PubMed
8. Yadav M, et al. 2008. J. Immunol. 180:2772. (Block) PubMed
9. Sintes J, et al. 2010. J. Leukoc. Biol. 88:687.  PubMed

Mouse myeloma FO cells stained

Mouse myeloma FO cells stained with D7715A7 APC



Description:

CD126 is an 80 kD IL-6 receptor α chain also known as IL-6R. It is a member of the immunoglobulin superfamily that is expressed on activated T and B cells, monocytes, hepatocytes, and plasma cells. High affinity IL-6 receptors are formed by the non-covalent association of CD126 and the IL-6 receptor β chain (CD130 or gp130). CD126 binds IL-6 with low affinity, but does not signal. The β chain (gp130, CD130) does not bind IL-6 by itself, but associates with the α-chain/IL-6 complex to initiate signal transduction. IL-6 binding to the receptor complex results in the stimulation of B and T cells, and hematopoietic precursor proliferation and differentiation. The D7715A7 (15A7) antibody blocks IL-6/IL-6 receptor interactions.

Other Names: IL-6 Receptor α chain, IL-6R
Structure: Ig superfamily, associates with IL-6Rβ chain (CD130, gp130), 80 kD
Distribution: Activated T cells and B cells, monocytes, hepatocytes, plasma cells
Function: Stimulates T cells, B cells, hematopoietic precursor proliferation and differentiation
Ligand Receptor: IL-6
Antigen References:

1. Taga T, et al. 1997. Annu. Rev. Immunol. 15:797.
2. Fitzgerald K, et al. 2001. The Cytokine FactsBook. Academic Press London.
3. Boulanger MJ, et al. 2003. Science 300:2101.