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Safety Data Sheet (SDS) Biotin Anti-mouse CD309 VEGFR2 Flk-1 Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse CD309 (VEGFR2, Flk-1) Antibody
1282045 50 µg $95.00       
Clone: Avas12
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Murine Flk1 fused to hIgG Fc
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Avas12 recognizes a different epitope than clone 89B3A5. Additional reported applications (for the relevant formats) include: Western blotting1 and immunohistochemical staining of paraformaldehyde-fixed frozen sections2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729. (WB)
2. Ishitobi H, et al. 2010. Exp. Anim. 59:615. (IHC)
3. Liu F, et al. 2015. EMBO Rep. 16:654. PubMed

bEnd.3 mouse endothelial cells stained

bEnd.3 mouse endothelial cells stained with biotinylated Avas12, followed by Sav-PE



Description:

CD309 is also known as vascular endothelial growth factor receptor 2 (VEGFR2) and fetal liver kinase-1 (Flk-1). CD309 is a member of the tyrosine protein kinase family that contains a single pass transmembrane receptor with a protein kinase domain and seven immunoglobulin-like domains in the extracellular region. CD309 is expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle. It's a receptor for VEGF or VEGFC, and plays an important role in the development of vascular endothelial cells, hematopoietic cells, and vascular permeability.

Other Names: Fetal liver kinase 1 (Flk-1), VEGF receptor-2, VEGF-R2, Ly-73, KDR
Structure: A receptor protein tyrosine kinase closely related to CD117 (c-kit) and CD140a (PDGF receptor α chain) of the immunoglobulin superfamily
Distribution: Expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle
Function: Plays an important role in the development of vascular endothelial cells, hematopoietic cells, and vascular permeability
Ligand Receptor: Vascular endothelial growth factor (VEGF)
Antigen References:

1. Kaburn N, et al. 1997. Development. 124:2039
2. Patterson C, et al. 1995. J. Bio. Chem. 270:23111
3. Nishikawa SI, et al. 1998. Immunity 8 (6):761
4. Shalaby F, et al. 1997. Cell. 89 (6):981