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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse CD205 DEC-205 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse CD205 (DEC-205) Antibody
1291015 25 µg $120.00       
1291020 100 µg $280.00       
Clone: NLDC-145
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse lymph node tissue
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IHC - Validated

Application Notes:

Additional reported applications (for relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1, Western Blot1-3, and immunoprecipitation of bone marrow dendritic cell extracts2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Application References:

1. Witmer-Pack MD, et al. 1995. Cell. Immun. 163:157. (IHC, WB)
2. Swiggard WJ, et al. 1995. Cell. Immun. 165:302. (WB, IP)
3. Bonifaz LC, et al. 2004. J. Exp. Med. 199:815. (WB)
4. Yamazaki S, et al. 2002. J. Immunol. 181:6923. (FC)
5. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC)

Bone marrow derived-dendritic cells from

Bone marrow derived-dendritic cells from C57BL/6 mouse stained with NLDC-145 Alexa Fluor® 647

C57BL/6 mouse bone marrow cells

C57BL/6 mouse bone marrow cells stained with Gr-1 (RB6-8C5) FITC and NLDC-145 Alexa Fluor® 647

C57BL/6 mouse bone marrow cells

C57BL/6 mouse bone marrow cells stained with Gr-1 (RB6-8C5) FITC and rat IgG2a Alexa Fluor® 647 isotype control



Description:

CD205, also known as DEC-205, is a 205 kD integral membrane protein homologous to the macrophage mannose receptor. It is a type I cell surface protein that belongs to the C-type lectin family.  CD205 is expressed at high levels by dendritic cells and thymic epithelial cells.  It is also expressed by a number of other cell types, such as B lymphocytes, macrophages, Langerhans cells, bone marrow stromal cells, granulocytes, epithelial cells of pulmonary airways, and the capillaries of the brain.  CD205 is a novel endocytic receptor used by dendritic cells and thymic epithelial cells to direct captured antigens from the extracellular space to specialized antigen processing.  It mediates antigen uptake and presentation and cross-presentation to T cells.  It has been reported that CD205 acts as a recognition receptor for dying cells, potentially provides an important pathway for the uptake of self-antigen in the intrathymic environment, and is involved in peripheral tolerance.  Antibody-mediated antigen-targeting via the DEC-205 receptor increases the efficiency of vaccination for T cell immunity.

Other Names: DEC-205
Structure: A 205 kD type I tramsmembrane protein belonging to the C-type lectin family and homologous to the macrophage mannose receptor.
Distribution: Expressed at high levels by dendritic cells and thymic epithelial cells. Also expressed by B lymphocytes, macrophages, Langerhans cells, bone marrow cells and epithelial cells.
Function: Mediates endocytosis, antigen uptake, presentation, and cross-presentation to T cells.
Antigen References:

1. Jiang WP, et al. 1995. Nature 375:151.
2. Small M and Kraal G. 2003. Int. Immunol. 15:197.
3. Shrimpton RE, et al. 2009. Mol. Immunol. 46:1229.


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