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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse Ly49D Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse Ly49D Antibody
1291530 100 µg $240.00       
Clone: 4E5
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for relevant formats) include: immunoprecipitation1 and blocking2, 3 of CHO cell or Con A-activated lymphoblast killing by NK cells.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Application References:

1. Mason LH, et al. 1998. J. Immunol. 160:4148. (IP)
2. Idris A, et al. 1999. Proc. Natl. Acad. Sci. 96:6330. (FC Block)
3. George TC, et al. 1999. J. Immunol. 162:2035. (FC Block)
4. Ortaldo JR, et al. 1999. J. Leukoc. Biol. 66:512. (FC)
5. Ortaldo JR, et al. 1998. J. Immunol. 160:1158. (FC)

C57BL/6 splenocytes stained with CD49b

C57BL/6 splenocytes stained with CD49b (DX5) PE and 4E5 Alexa Fluor® 647

C57BL/6 splenocytes stained with CD49b

C57BL/6 splenocytes stained with CD49b (DX5) PE and rat IgG2a Alexa Fluor® 647



Description:

Ly49D is a type II transmembrane protein belonging to the Ly49 family of NK-cell receptors in the C-type lectin superfamily.  Ly49D is expressed on subsets of natural killer (NK) cells in C57BL/6, C3H/He and SJL mouse strains, but not Balb/c, DBA/2, AKR and CBA/J.  The receptors form homodimers that bind to MHC class I alloantigens.  Unlike inhibitory Ly49 family members, Ly49D lacks a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) but contains a cytoplasmic immunoreceptor tyrosine-based activation motif.  The activating receptor Ly49D delivers stimulatory signals for target cell lysis upon interacting with H2-Dd, Dr and Dsp2, but not with H2b or H2k class I antigens.  Cross-linking of Ly49D activates intracellular kinase activity and calcium mobilization in NK cells.  In 129/J mice, 4E5 antibody cross-reacts with Ly49O, LY49R and Ly49V.

Other Names: Ly-49D
Structure: A type II transmembrane protein belonging to the Ly49 family of NK-cell receptors in the C-type lectin superfamily.
Distribution: Subsets of natural killer (NK) cells in C57BL/6, C3H/He and SJL, but not Balb/c, DBA/2, AKR and CBA/J.
Function: Plays an important role in activating natural killer (NK) cell cytolytic activity.
Ligand Receptor: MHC class I alloantigens
Antigen References:

1. Yokoyama WM and Seaman WE. 1993. Annu. Rev. Immunol. 11:613.
2. Takei F, et al. 1997. Immunol. Rev. 155:67.
3. Hanke T, et al. 1999. Immunity 11:67.
4. Mason LH, et al. 1996. J. Exp. Med. 184:2119.


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