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Safety Data Sheet (SDS) PE Anti-mouse Ly-6D Antibody     Product Data Sheet (PDF)    
PE Anti-mouse Ly-6D Antibody
1293015 25 µg $90.00       
1293020 100 µg $225.00       
Clone: 49-H4
Isotype: Rat IgG2c, κ
Reactivity: Mouse
Immunogen: BALB/c mouse plasmacytoma MOPC-104E
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Eckhardt LA and Herzenberg LA. 1980. Immunogenetics 11:275. (FC)

IgM- splenocytes from C57BL/6 mouse

IgM- splenocytes from C57BL/6 mouse stained with 49H4.3 PE and CD43 FITC

IgM- splenocytes from C57BL/6 mouse

IgM- splenocytes from C57BL/6 mouse stained with isotype control PE and CD43 FITC



Description:

Lymphocyte antigen 6D (Ly-6D), also known as thymocyte B-cell antigen (ThB), is a 16 kD GPI-linked cell surface glycoprotein. It belongs to the Ly6 gene family and contains one urokinase receptor (uPAR) /Ly6 domain. Ly-6D is involved in cell-cell adhesion and signal transduction. Ly-6D marks the earliest stage of B cell lineage commitment from the common lymphoid progenitors (CLP). It is expressed on B cells, immature thymocytes, and plasmacytoid dendritic cells.

Other Names: Lymphocyte antigen 6D, Thymocyte B-cell antigen, ThB
Structure: GPI-linked cell-surface glycoprotein, molecular weight 16 kD, belongs to the Ly6 gene family, contains one urokinase receptor (uPAR) /Ly6 domain
Distribution: B cells, B cell-biased lymphoid progenitor (BLP), thymocytes, plasmacytoid dendritic cells
Function: Cell-cell adhesion and signal transduction
Antigen References:

1. Inlay MA, et al. 2009. Genes Dev. 23:2376.
2. Gumley TP, et al. 1995. Immunol. Cell Biol. 73:277.
3. Mansson R, et al. 2010. Blood 115:2601.
4. Reese JT, et al. 2001. Dev. Immunol. 8:107.