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Safety Data Sheet (SDS) PE Anti-mouse Ly-49AB6 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse Ly-49AB6 Antibody
1293515 25 µg $75.00       
Clone: A1/Ly49A
Isotype: Mouse IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse C57BL/6N T lymphoma EL-4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Nagasawa R, et al. 1987. J. Immunol. 138:815. (IP)
2. Guy-Grand D, et al. 1996. Eur. J. Immunol. 26:2248. (FC)
3. Zimmer J, et al. 2001. J. Exp. Med. 194:1531. (FC)

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with A1/Ly49A PE and NK1.1 APC



Description:

Ly-49A is a 44 kD member of the C-type lectin/Ly-49 family proteins. It is a type-II transmembrane, disulfide-linked homodimer with C-type lectin extracellular domains. Ly-49A is present on a subset of NK and NK-T cells. Ly-49A, like other members of this family, inhibits NK cell activation through the recognition and interaction of certain MHC class I haplotypes on potential target cells. The Ly-49A receptor recognizes H-2Dd and H-2Dk, triggering phosphorylation of the ITIM motif in the cytoplasmic domain and recruitment of SHP-1 and SHP-2. The A1/Ly49A antibody preferentially reacts with CRD of Ly-49AB6 alloantigen and has also shown cross-reaction with Ly-49ANOD, Ly-49PNOD and Ly-49P129/J.

Other Names: Ly49A
Structure: C-type lectin, Ly-49 family, ITIM, 44 kD
Distribution: NK cells, NKT cells
Function: Inhibitory NK cell receptor
Ligand Receptor: H-2Dd, H-2Dk, H-2Dp
Antigen References:

1. Coles MC, et al. 2000. Eur. J. Immunol. 30:236.
2. Takei FJ, et al. 1997. Immunol. Rev. 155:67.
3. Hanke T, et al. 1999. Immunity 11:67.
4. Silver ET, et al. 2000. J. Immunol. 165:1771.