The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1,2 and in vitro activation of NK cells1.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Walzer T, et al. 2007. P. Natl. Acad. Sci. USA 104:3384. (FC, Activ) 2. Walzer T, et al. 2007. Nat. Immunol. 8:1337. (FC, Activ) 3. Guerriero JL, et al. 2011. J. Immunol. 186:3517. (IHC) PubMed
C57BL/6 splenocytes stained with NK1.1 (PK136) APC and 29A1.4 FITC
C57BL/6 splenocytes stained with NK1.1 (PK136) APC and rat IgG2a, κ FITC isotype control
CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.
Single-pass type I membrane protein, 46 kD; belongs to the natural cytotoxicity receptor (NCR) family; contains 2 Ig-like (immunoglobulin-like) domains
Mature and immature NK cells, subset of NKT cells, but not on CD1d-restricted NKT cells
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