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Safety Data Sheet (SDS) PE Anti-mouse TCR Vbeta11 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse TCR Vβ11 Antibody
1295015 25 µg $85.00       
1295020 100 µg $175.00       
Clone: RR3-15
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: Mouse cytolytic T-cell clone OH6
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for relevant formats) include: Western Blot1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Bill J, et al. 1989. J. Exp. Med. 169:1405. (WB)

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with CD3 (145-2C11) APC and RR3-15 PE

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with CD3 (145-2C11) APC and rat IgG2b, κ (RTK4530) PE isotype control



Description:

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex.  The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.  V β 11-bearing T cells are largely absent from strains of mice that express MHC class II molecule I-E.  It has been demonstrated that the expression of I-E molecule is necessary for the clonal deletion of V beta 11-bearing T cells, but not sufficient for the clonal elimination of V beta 11-bearing T cells.

Other Names: TCR Vβ-11, V beta 11 TCR, Vβ11 T-cell receptor,
Structure: A member of Ig superfamily, heterodimerized with α TCR α chain, and associated with CD3 complex.
Distribution: Subsets of T cells and thymocytes.
Function: Antigen recognition.
Ligand Receptor: Antigens
Antigen References:

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Kubo R, et al. 1989. J. Immunol. 142:2736.
3. Haqqi TM, et al. 1989. J. Exp. Med. 169:1903.
4. Behlke MA, et al. 1986. Proc. Natl. Acad. Sci. USA 83:767.
5. Gao EK, et al. 1989. J. Exp. Med. 170:1947.