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Safety Data Sheet (SDS) Pacific Blue Anti-mouse I-Ab Antibody     Product Data Sheet (PDF)    
Pacific Blue™ Anti-mouse I-Ab Antibody
1182105 25 µg $95.00       
1182110 100 µg $215.00       
Clone: AF6-120.1
Isotype: Mouse (BALB/c) IgG2a, κ
Reactivity: Mouse b haplotype, cross-reacts with H-2k and H-2u haplotypes. Does not react with other haplotypes (e.g., d, f, q, r, s).
Immunogen: C57BL/10J splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

 Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of frozen sections (acetone-fixed5; OCT-embedded, ethanol-fixed sections7), immunofluorescence microscopy3 (including acetone-fixed epidermal sheets6), immunoprecipitation7,8. Directly conjugated antibody was used for IF in (3) and (6) and for IHC in (5).

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Application References:

1. Wall KA, et al. 1983. J. Immunol. 131:1056. (FC)
2. Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747. (FC)
3. Inaba K, et al. 1998. J. Exp. Med. 188:2163 (IF)
4. Hamrah P, et al. 2002. Invest Opthalmol Vis. Sci. 43:639 (IF)
5. Buono C, et al. 2003. Arterioscler. Thromb. Vasc. Biol. 23:454. (IHC)
6. Wang Z, et al. 2004. J. Immunol. 172:5924. (IHC IF)
7. Nakagawa TY, et al. 1999. Immunity 10:207. (IP)
8. Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC IP) PubMed
9. Tay SS, et al. 2014. J Immunol. 193:2087. PubMed

C57BL/6 splenocytes stained with AF6-120.1

C57BL/6 splenocytes stained with AF6-120.1 Pacific Blue™



Description:

The AF6-120.1 antibody reacts with the I-Ab MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b bearing mice, and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The AF6-120.1 antibody cross-reacts with H-2k and H-2u haplotypes; this antibody does not cross-react with other haplotypes (d, f, q, r, s).

Other Names: MHC class II
Structure: MHC class II
Distribution: B cell and activated T cells, APCs of H-2b mice
Function: Antigen presentation
Ligand Receptor: CD3/TCR, CD4
Antigen References:

1. Watts C. 1997. Annu. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323.


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.