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Safety Data Sheet (SDS) APC Anti-mouse Podoplanin Antibody     Product Data Sheet (PDF)    
APC Anti-mouse Podoplanin Antibody
1237045 25 µg $110.00       
1237050 100 µg $250.00       
Clone: 8.1.1
Isotype: Syrian Hamster IgG
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemistry6.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
2. Farr AG, et al. 1992. J. Exp. Med. 176:1477.
3. Bekiaris V, et al. 2008. J. Immunol. 180:6768.
4. Algars A, et al. 2011. Blood 117:4387. PubMed
5. Reis VO, et al. 2012. Immunobiology. 217:831. PubMed
6. Kaji C, et al. 2012. Acta. Histochem. Cytochem. 45:227. (IHC)
7. Kretschmer S, et al. 2013. PLoS One. 8:e52201. PubMed.

Mouse thymic epithelial stromal cell

Mouse thymic epithelial stromal cell line TE-71 stained with 8.1.1 APC



Description:

The mucin-type glycoprotein podoplanin is thought to be involved in the development of the lymphatic vascular system. Podoplanin is named after its expression in the kidney glomerular epithelial cells (podocytes). It has a potential role in tumor progression.

Other Names: T1a, gp38, stromal cell marker
Structure: 43 Kd glycosylated type-1 transmembrane protein. Mucin-type protein.
Distribution: Expressed on epithelial and mesothelial cells.
Antigen References:

1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
2. Schacht V, et al. 2005. Am. J. Pathol. 166:913.