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Safety Data Sheet (SDS) PerCP/Cy55 Anti-mouse CD31 Antibody     Product Data Sheet (PDF)    
PerCP/Cy5.5 Anti-mouse CD31 Antibody
1112095 25 µg $100.00       
1112100 100 µg $315.00       
Clone: 390
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application References:

1. Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
2. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
3. Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
4. Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
5. Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
6. Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
7. McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
8. Chacko AM, et al. 2012. PLoS One 7:e34958. 
9. Noll JE, et al. 2014. Haematologica. 99:163. PubMed

C57BL/6 splenocytes stained with 390

C57BL/6 splenocytes stained with 390 PerCP/Cy5.5



Description:

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.

Other Names: PECAM-1, EndoCAM
Structure: Ig superfamily, 130-140 kD
Distribution: Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets
Function: Adhesion
Ligand Receptor: CD38, αV3 integrin
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
COMMERCIAL USE shall include:

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  • use of this material to perform services for a fee for third parties.

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