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Safety Data Sheet (SDS) APC Anti-mouse CD279 PD-1 Antibody     Product Data Sheet (PDF)    
APC Anti-mouse CD279 (PD-1) Antibody
1145555 25 µg $95.00       
1145560 100 µg $270.00       
Clone: RMP1-30
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: Mouse PD-1 transfected BHK cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported application (for the relevant formats) include: Functional assay.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

1. Matsumoto K, et al. 2004. J. Immunol. 172:2530.
2. Raimondi G, et al. 2006. J. Immunol. 176:2808. (FC) PubMed
3. King IL, et al. 2009. J. Exp Med 206:1001. (FC) PubMed

Con A-stimulated C57BL/6 splenocytes (Day

Con A-stimulated C57BL/6 splenocytes (Day 3) stained with RMP1-30 APC



Description:

CD279 is a 50-55 kD immunoglobulin superfamily member also known as programmed death-1 (PD-1). PD-1 is expressed on a subset of CD4-CD8- thymocytes and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily.

Other Names: Programmed Death-1, CD279
Structure: Ig superfamily, 50-55 kD
Distribution: Subset of double negative thymocytes, activated T and B cells
Function: Lymphocyte clonal selection, peripheral tolerance
Ligand Receptor: PD-L1 (B7-H1), PD-L2
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Agata Y, et al. 1996. Int. Immunol. 8:765.
3. Nishimura H, et al. 2001. Science 291:319.
4. Ishida Y, et al. 1992. EMBO J. 11:3887.