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Safety Data Sheet (SDS) PE Anti-mouse CD64 FcgammaRI Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD64 (FcγRI) Antibody
1296515 25 µg $100.00       
1296520 100 µg $245.00       
Clone: X54-5/7.1
Isotype: Mouse IgG1, κ
Reactivity: Mouse CD64 a and b alloantigens.
Immunogen: BALB/c mouse FcγRI-human IgG Fc fusion protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1. Clone X54-5/7.1 is not found to be useful for Western blots1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Tan PS, et al. 2003. J. Immunol. 170:2549. (IP)
2. Ingersoll MA, et al. 2010. Blood 115:e10. (FC)
3. Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed
4. Parsons MW, et al. 2014. J. Immunol. 192:1361. PubMed
5. Conquery CM, et al. 2014. PLoS One. 9:102284. PubMed
6. Lee MR, et al. 2014. PLoS One. 9:112666. PubMed
7. van Blijswijk J, et al. 2015. J Immunol. 194:307. PubMed
8. Karsten CM, et al. 2015. J Immunol. 194:1841. PubMed
9. Stijlemans B, et al. 2015. PLoS Negl Trop Dis. 9:3561. PubMed
10. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed
11. Cnops J, et al. 2015. PLoS Pathog. 11:1004964. PubMed

C57BL/6 bone marrow cells stained

C57BL/6 bone marrow cells stained with CD11b FITC and X54-5/7.1 PE

C57BL/6 bone marrow cells stained

C57BL/6 bone marrow cells stained with CD11b FITC and mouse IgG1 PE isotype control



Description:

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Other Names: FcRI
Structure: Ig superfamily, type I glycoprotein, 72 kD
Distribution: Monocytes, macrophages, mast cells, dendritic cells
Function: Phagocytosis, ADCC
Ligand Receptor: IgG