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Safety Data Sheet (SDS) PE Anti-mouse B7-H4 B7S1 B7X Antibody     Product Data Sheet (PDF)    
PE Anti-mouse B7-H4 (B7S1, B7X) Antibody
1297025 25 µg $115.00       
1297030 100 µg $310.00       
Clone: HMH4-5G1
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Mouse B7-H4 human IgG1 Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported application (for relevant formats) include: blocking1.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

1. Personal communication (Block)

Mouse B7-H4 transfected 293 cells

Mouse B7-H4 transfected 293 cells stained with HMH4-5G1 PE



Description:

B7-H4 is a newly discovered B7 family member that negatively regulates T cell immunity. In vitro, B7-H4 inhibits CD4+ and CD8+ T cell proliferation, cytokine production, and generation of alloreactive cytotoxic T-cells (CTLs). In vivo, blockage of endogenous B7-H4 by specific monoclonal antibodies promotes T cell responses. A new reported function of B7-H4 is as an important negative regulator of innate immunity through growth inhibition of neutrophils. It has been reported that B7-H4 is not constitutively expressed on peripheral tissues but can be induced to express on T cells, B cells, macrophages, and dendritic cells. B7-H4 is expressed on some tumor cancer cells. The role of B7-H4 in tumor progression may be to transform precancerous cells and then protect them from immunosurveillance. Although B- and T-lymphocyte attenuator (BTLA) was proposed to be the receptor for B7-H4, further studies are needed to identify the inhibitory receptor of B7-H4.

Other Names: B7H4, B7S1, B7-S1, B7x
Structure: Belongs to the B7 family, contains two extracellular Ig-like domains.
Distribution: Induced to express on T cells, B cells, macrophages, and dendritic cells. It is also expressed on some tumor cancer cells.
Function: Inhibit T cell activation, cell cycle progression, and cytokine production.
Antigen References:

1. Choi IH, et al. 2003. J. Immunol. 171:4650.
2. Prasad DV, et al. 2003. Immunity 18:863.
3. Kryczek I, et al. 2006. J. Exp. Med. 203:871.