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Safety Data Sheet (SDS) PE Anti-mouse TCR Vbeta51 52 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse TCR Vβ5.1, 5.2 Antibody
1297515 25 µg $105.00       
1297520 100 µg $280.00       
Clone: MR9-4
Isotype: Mouse IgG1, κ
Reactivity: Mouse
Immunogen: Murine T cell hybridoma 2HB51.8
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: Induction of proliferation of Vβ5.1+ and Vβ5.2+ T cells2, 3 and in vivo depletion of Vβ5+ T cells4.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Kanagawa O, et al. 1991. J. Immunol. 147:1307. (FC)
2. Kanagawa O, et al. 1992. J. Immunol. 149:9. (Activ)
3. Woodland DL, et al. 1993. J. Exp. Med. 177:433. (Activ)
4. Gelber C, et al. 1992. Cancer Res. 52:6507. (Deplete)

C57BL/6 splenocytes stained with CD3e

C57BL/6 splenocytes stained with CD3e (145-2C11) APC and MR9-4 PE

C57BL/6 splenocytes stained with CD3e

C57BL/6 splenocytes stained with CD3e (145-2C11) APC and mouse IgG1 PE isotype control



Description:

Vβ5.1 and 5.2 T cell receptor (TCR Vβ5.1, 5.2) are variants of TCR β chain that, along with TCR α chain, forms the TCR heterodimer. In association with the CD3 complex, TCR α/β is responsible for antigen recognition in the MHC-Peptide complex and the initiation of T cell-mediated immune responses.

Other Names: Vβ5 T-cell receptor, T cell receptor beta 5
Structure: Member of the immunoglobulin superfamily, Vβ5.1and 5.2 are variants of the TCR β chain which associates with the TCR α chain to form the TCR heterodimer.
Distribution: Expressed on a subset of TCR αβ+ T cells.
Function: Recognition of peptides presented by the MHC molecules, responsible for T cell mediated immune responses.
Ligand Receptor: MHC-Peptide complex
Antigen References:

1. Marrack P, et al. 2008. Annu. Rev. Immunol. 26:171.
2. Sim GK and Augustin AA. 1985. Cell 42:89.
3. Mami-Chouaib F, et al. 2002. Immunol. Rev. 188:114.