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Safety Data Sheet (SDS) PE Anti-mouse MAIR-IV CLM-5 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse MAIR-IV (CLM-5) Antibody
1298025 25 µg $115.00       
Clone: TX69
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: MAIR-IV transfected BW5147 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: Western blot1, plate-coated TX69 induced secretion of IL-6, and TNF-α by neutrophils1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Nakano T, et al. 2008. Mol. Immunol. 45:289. (Activ, WB)

Mouse MAIR-IV transfected cells stained

Mouse MAIR-IV transfected cells stained with TX-69 PE



Description:

MAIR-IV, also known as LMIR4 or CLM5, is a member of the myeloid-associated immunoglobulin-like receptor family (MAIR, CD300). It is preferentially expressed on Gr-1+/Mac-1+ neutrophils in the peripheral blood, bone marrow, peritoneal cavity, and spleen. It is also found on Mac-1+/Gr-1+ macrophages and CD11c+ dendritic cells. In association with the immunoreceptor tyrosine based activating motif (ITAM)-bearing adaptor FcRγ, MAIR-IV mediates activation signals for neutrophils.

Other Names: LMIR4
Structure: Ig superfamily, associates with ITAM-bearing adaptor FcRγ
Distribution: Neutrophils, macrophages, dendritic cells
Function: Activates neutrophils
Antigen References:

1. Shibuya A, et al. 2009. Immune Network 9:41.
2. Can I, et al. 2008. J. Immunol. 180:207.
3. Nakano T, et al. 2008. Mol. Immunol. 45:289.