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Safety Data Sheet (SDS) PE Anti-mouse TCR Vbeta6 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse TCR Vβ6 Antibody
1300015 25 µg $100.00       
1300020 100 µg $215.00       
Clone: RR4-7
Isotype: Rat IgG2b, λ
Reactivity: Mouse
Immunogen: H-Y specific TH Clone OI11
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: induction of T cell proliferation1, blocking in vitro cytolysis1, and in vivo blocking of syngeneic T cell tumor growth2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Kanagawa O and Palmer EBJ. 1989. Cell. Immunol. 119:412. (Activ, Block)
2. Kanagawa O. 1989. J. Exp. Med. 170:1513. (Block)

C57BL/6 splenocytes stained with RR4-7

C57BL/6 splenocytes stained with RR4-7 PE and CD3 APC



Description:

Vβ6 T cell receptor (TCR Vβ6) is a variant of TCR β chain and, along with TCR α chain, forms the TCR heterodimer. In association with the CD3 complex, TCR α/β is responsible for antigen recognition in the MHC-Peptide complex and the initiation of the T cell-mediated immune responses. Murine Vβ6 cells recognize the Mtv-7, Mls-1, and rabies superantigens.

Other Names: T cell receptor beta 6
Structure: Member of the immunoglobulin superfamily, Vβ6 is a variant of the TCR β chain which associates with the TCR α chain to form the TCR heterodimer.
Distribution: Expressed on a subset of TCR αβ+ T cells.
Function: Recognition of peptides presented in the MHC molecules, responsible for T cell mediated immune responses.
Ligand Receptor: MHC-Peptide complex
Antigen References:

1. Acha-Orbea H and MacDonald HR. 1995. Annu. Rev. Immunol. 13:459.
2. Rudy CK, et al. 1992. J. Exp. Med. 175:1613.
3. Jones LA, et al. 1990. Science 250:1726.