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Safety Data Sheet (SDS) Purified Anti-mouse TCR Vbeta81 82 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse TCR Vβ8.1, 8.2 Antibody
1300510 100 µg $180.00       
Clone: MR5-2
Isotype: Mouse IgG2a, κ
Reactivity: Mouse
Immunogen: H-Y specific TH Clone OI6
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IHC - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: induction of proliferation of Vβ8.1+ and Vβ8.2+ T cells1,2 and immunohistochemical staining3.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Kanagawa OJ. 1988. J. Immunol. Methods 110:169. (Activ)
2. Denkers EY, et al. 1986. J. Immunol. 156:1089. (Activ)
3. Drescher KM, et al. 2000. Int. Immunol. 12:271. (IHC)

BALB/c splenocytes stained with purified

BALB/c splenocytes stained with purified MR5-2 conjugated with PE and CD3 APC



Description:

Vβ8 T cell receptor (TCR Vβ8) subfamily has three members, Vβ8.1, 8.2, and 8.3. These subfamily members are variants of the TCR β chain that along with the TCR α chain forms the TCR heterodimer, responsible for the recognition of peptides presented by the MHC molecules. Murine Vβ8+ T cells recognize the superantigen staphylococcal enterotoxin B (SEB); in vivo administration of SEB results in the negative selection of Vβ8+ cells in the thymus. Vβ8 is one of the most frequently found β chains on the infiltrating T cells in the brain of mice with experimental autoimmune encephalomyelitis (EAE), and on the T cells response to Toxoplasma gondii.

Other Names: T cell receptor beta 8
Structure: Members of the immunoglobulin superfamily, Vβ8.1 and 8.2 are variants of the TCR β chain which associates with the TCR α chain to form the TCR heterodimer.
Distribution: Expressed in a subset of TCR αβ+ T cells
Function: Recognition of peptides presented in the MHC molecules, responsible for T cell mediated immune response
Ligand Receptor: MHC-Peptide complex
Antigen References:

1. Marrack P, et al. 2008. Annu. Rev. Immunol. 26:171.
2. Acha-Orbea H and MacDonald HR. 1995. Annu. Rev. Immunol. 13:459.
3. Sim GK and Augustin AA. 1985. Cell 42:89.
4. Mami-Chouaib F, et al. 2002. Immunol. Rev. 188:114.