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Safety Data Sheet (SDS) FITC Anti-mouse CD8b2 Antibody     Product Data Sheet (PDF)    
FITC Anti-mouse CD8b.2 Antibody
1302015 50 µg $55.00       
1302020 500 µg $195.00       
Clone: 53-5.8
Isotype: Rat IgG1, κ
Reactivity: Mouse
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunofluorescence3, immunohistochemical staining4 of frozen tissue section using dry ice-isopentane method and immunoprecipitation5.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Ledbetter J, et al. 1979. Immunol. Rev. 47:63.
2. Ledbetter J, et al. 1980. J. Exp. Med. 152:280.
3. Vremec D, et al. 2000. J. Immunol. 164:2978. (IF)
4. Lawrence D, et al. 1999. J. Virol. 73:1795. (IHC)
5. Bosselut R, et al. 1999. J. Exp. Med. 190:1517. (IP)
6. Das DK, et al. 2015. PNAS. 112:1517. PubMed

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with CD8b.2 (53-5.8) FITC and CD8a (53-6.7) APC



Description:

CD8b is the 32 kD β chain of CD8, also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation. The antibody 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1.

Other Names: Lyt-3.2, Ly-3.2
Structure: Ig superfamily, CD8β chain, 32 kD
Distribution: majority of thymocytes, cytotoxic T cell subsets
Function: Co-receptor of TCR
Ligand Receptor: MHC class I
Antigen References:

1. Ledbetter J, et al. 1981. J. Exp. Med. 153:1503.
2. Renard V, et al. 1996. J. Exp. Med. 184:2439.