The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Dent AL, et al. 1990. Nature 343:714. 2. Kelly KA, et al. 1993. Int. Immunol. 5:331. 3. Sperling AI, et al. 1992. J. Immunol. 149:3200. 4. Sperling AI, et al. 1997. J. Immunol. 159:86.
C57BL/6 mouse lymphocytes stained with TCR VÎ³2 (UC3-10A6) PE and CD3 (145-2C11) APC
T cell receptor (TCR) Vγ2 bearing T lymphocytes make up a significant proportion of γδ TCR cells in late fetal and adult peripheral lymphoid tissues. TCR γδ T cells may play a role in immunological surveillance for stress-induced self-antigens. The frequency of Vγ2 expression in different strains varied from 12% to 54% in the TCR γδ repertoire. Variations in the levels of Vγ2+ cells are not associated with MHC haplotype. High Vγ2 expression is influenced by the TCR-δ locus. Expanding Vγ2+ TCRγδ cells in B6 mice overwhelmingly use a Vδ7+ δ chain except in the DBA/2 strain.
Vγ2 T-cell receptor, T cell receptor gamma 2
A member of Ig superfamily, associated with TCR δ chain and CD3 complex.
T cell subset in fetal and adult peripheral lymphoid tissues and thymus.
Might play a role in a previously unrecognized form of immunological surveillance for stress-induced self-antigens.
1. Allison JP, et al. 1991. Annu. Rev. Immunol. 9:679. 2. O’Brien RL, et al. 2000. J. Immunol. 165:6472.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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