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Safety Data Sheet (SDS) PerCP Anti-mouse CD902 Thy12 Antibody     Product Data Sheet (PDF)    
PerCP Anti-mouse CD90.2 (Thy1.2) Antibody
1301575 25 µg $95.00       
1301580 100 µg $245.00       
Clone: 53-2.1
Isotype: Rat IgG2a, κ
Reactivity: Mouse, Does not react with Thy-1.1 (CD90.1)
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP under optimal conditions. The solution is free of unconjugated PerCP and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining1 of frozen tissue section, immunofluorescence2, and immunoprecipitation3.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Application References:

1. Aldrich M, et al. 2003. J. Immunol. 171:5562. (IHC)
2. Jameson J, et al. 2004. J. Immunol. 172:3573. (IF)
3. Okada C, et al. 1990. J. Immunol. 144:3473. (IP)

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with CD90.2 (53-2.1) PerCP



Description:

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy-1.2, a GPI-linked membrane molecule. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45.

Other Names:
Structure: Ig superfamily, 25-35 kD
Distribution: Hematopoietic stem cells and neurons, epithelial cells, fibroblasts, neurons, all thymocytes, and peripheral T cells of Thy-1.2 bearing mice
Function: Lymphocyte costimulation, proliferation, and differentiation of hematopoietic stem cells
Ligand Receptor: CD45
Antigen References:

1. Borrello M, et al. 1996. Cell. Immunol.173:198.
2. Radrizzani M, et al. 1995. J. Neurosci. Res. 42:220.
3. Williams A, et al. 1982. Science 216:696.