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Safety Data Sheet (SDS) PerCP/Cy55 Anti-mouse FcepsilonRIalpha Antibody     Product Data Sheet (PDF)    
PerCP/Cy5.5 Anti-mouse FcεRIα Antibody
1271595 25 µg $150.00       
1271600 100 µg $320.00       
Clone: MAR-1
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for relevant formats of this clone) include: depletion2, immunohistochemistry of frozen sections (OCT embedded2).

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application References:

1. Obata K, et al. 2007. Blood 110:913 (FC)
2. Sokol CL, et al. 2008. Nat. Immunol. 9:310 (FC, Deplete, IHC)
3. Chen J, et al. 2009. J. Biol. Chem.. 284:5763 (FC)

Mouse mast cell line MC/9

Mouse mast cell line MC/9 stained with MAR-1 PerCP/Cy5.5



Description:

FcεRIα is a transmembrane protein belonging to the Ig superfamily. FcεRIα forms a tetrameric complex with one β and two γ-subunits. The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions. It is abundantly expressed on mast and basophils and up-regulated by the presence of IgE. Following stimulation via FcεRIα, mast cells and basophils release bioactive chemical mediators such as histamine, resulting in the initiation of allergic reactions. Cross linking of the high-affinity receptor for IgE on tissue mast cells triggers immediate hypersensitivity with local symptoms. The MAR-1 monoclonal antibody reacts with the FcεRIα subunit.

Other Names: FceRIa, FceRI-α, high affinity IgE receptor
Structure: A transmembrane protein of Ig super family member
Distribution: Expressed on mast and basophils.
Function: Play an important role in triggering IgE-mediated allergic reactions.
Ligand Receptor: IgE
Antigen References:

1. Arinobu Y, et al. 2005. P. Natl. Acad. Sci. USA 102:18105.
2. Yamaguchi M, et al. 2001. Int. Immunol. 13:843.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
COMMERCIAL USE shall include:

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  • use of this material to perform services for a fee for third parties.

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