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Safety Data Sheet (SDS) PE Anti-mouse TCR Vbeta13 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse TCR Vβ13 Antibody
1303515 25 µg $100.00       
1303520 100 µg $215.00       
Clone: MR12-4
Isotype: Mouse IgG1, κ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: in vitro blocking of antigen-induced proliferation of Vβ13+ T cells 1 and in vivo depletion of Vβ13+ T cells1.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Zaller DM, et al. 1990. J. Exp. Med. 171:1943. (Block)

BALB/c splenocytes stained with MR12-4

BALB/c splenocytes stained with MR12-4 PE and CD3 APC



Description:

Vβ13 T cell receptor (TCR Vβ13) is a variant of TCR β chain that along with TCR α chain forms the TCR heterodimer. In association with the CD3 complex, TCR α/β is responsible for antigen recognition in the MHC-Peptide complex and initiation of T cell-mediated immune responses. The most frequently β chains found in the immune response to myelin basic protein (MBP) in B10.PL mice are Vβ13 and Vβ8.2.

Other Names: Vβ13 T-cell receptor, T cell receptor beta 13
Structure: Member of the immunoglobulin superfamily, Vβ13 is a variant of the TCR β chain which associates with the TCR α chain to form the TCR heterodimer.
Distribution: Expressed on a subset of TCR αβ+ T cells.
Function: Recognition of peptides presented by the MHC molecules, responsible for T cell mediated immune responses.
Ligand Receptor: MHC-Peptide complex
Antigen References:

1. Zaller DM, et al. 1990. J. Exp. Med. 171:1943.
2. Gentile F, et al. 2004. Immunology 112:13.
3. Lode HN, et al. 1999. P. Natl. Acad. Sci. USA 96:8591.