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Safety Data Sheet (SDS) APC Anti-mouse LPAM-1 Integrin Alpha4beta7 Antibody     Product Data Sheet (PDF)    
APC Anti-mouse LPAM-1 (Integrin α4β7) Antibody
1203035 25 µg $95.00       
1203040 100 µg $265.00       
Clone: DATK32
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: TK1 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell adhesion1-4, in vitro induction of TK1 cell aggregation1, immunoprecipitation4, and immunohistochemical staining of frozen sections5. It has been reported that the DATK32 mAb is able to block α4β7 mediated lymphocyte adhesion to VCAM-1, MAdCAM-1, and fibronectin in vitro and in vivo

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block)
2. Berlin C, et al. 1993. Cell 74:185. (Block)
3. Berlin-Rufenach C, et al. 1999. J. Exp. Med. 189:1467. (Block)
4. Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343. (Block, IP)
5. Bogetto L, et al. 2000. Blood 95:2397. (IHC)

C57BL/6 splenocytes stained with DATK32

C57BL/6 splenocytes stained with DATK32 APC

C57BL/6 mouse bone marrow cells

C57BL/6 mouse bone marrow cells were surface stained with B220 PE and LPAM-1 APC (clone DATK32) (top) or rat IgG2a, κ APC isotype control (bottom).





Description:

DATK32 antibody is specific for a combinatorial determinate of integrin α4β7complex. Integrin α4β7 is composed of a 150 kD (α4 or CD49d) and a 130 kD (β7) heterodimer, also known as CD49d/β7 or LPAM-1. Belonging to the Ig superfamily, it is found on the majority of peripheral lymphocytes and subsets of thymocytes and bone marrow cells (including mast cell progenitors). Integrin α4β7 binds its ligands, VCAM-1 (CD106), MAdCAM-1 and fibronectin, and plays an important role in lymphocytes adhesion and the direction of migration of blood lymphocytes to the intestine and associated lymphoid tissues.

Other Names: α4β7 Integrin, CD49d/β7, LPAM-1, ITGA4, ITGB7
Structure: Integrin family, Ig superfamily, 150 kD/130 kD heterodimer
Distribution: Majority of peripheral lymphocytes, subsets of thymocytes and bone marrow cells
Function: Lymphocyte adhesion
Ligand Receptor: VCAM-1 (CD106), MAdCAM-1, fibronectin
Antigen References:

1. Andrew DP, et al. 1994. J. Immunol. 153:3847.
2. Berlin C, et al. 1994. Cell 74:185.
3. Gurish MF, et al. 2001 J. Exp. Med. 194:1243.
4. Hamann A, et al. 1994. J. Immunol. 152:3282.