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Safety Data Sheet (SDS) PE/Cy7 Anti-mouse/rat/human CD27 Antibody     Product Data Sheet (PDF)    
PE/Cy7 Anti-mouse/rat/human CD27 Antibody
1221075 25 µg $95.00       
1221080 100 µg $270.00       
Clone: LG.3A10
Isotype: Armenian Hamster IgG
Reactivity: Mouse, Rat, Human
Immunogen: Armenian hamster fibroblast line ARHO12 transfected with mouse CD27 cDNA
Formulation: Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: functional assay1,3 and immunohistomecial staining of acetone-fixed frozen sections2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Gravestein LA, et al. 1995. Int. Immunol. 7:551. (FA)
2. Gravestein LA, et al. 1996. J. Exp. Med. 184:675. (IHC)
3. Takeda K, et al. 2000. J. Immunol. 164:1741. (FA)
4. Welner RS, et al. 2009. J. Immunol. 183:7768. PubMed
5. Vicetti Miguel RC, et al. 2012. J. Immunol. 189:3449. PubMed
6. White CA, et al. 2014. J Immunol. 193:5933. PubMed

C57BL/6 mouse thymocytes stained with

C57BL/6 mouse thymocytes stained with LG.3A10 PE/Cy7



Description:

CD27 is also known as S152 and T14. A member of the tumor necrosis factor receptor (TNFR) superfamily, it is a 45 kD protein expressed on peripheral T cells, memory B cells, NK cells, and thymocyte subset. Through its ligand, CD70, CD27 plays a key role in T cell and B cell interactions. Additionally, ligation of CD27 on naïve T cells may be important in their maturation to effector cells.

Other Names: T14, S152, Tp55, TNFRSF7
Structure: TNFR superfamily, 45 kD
Distribution: Peripheral blood T cells, memory B cells, NK cells, thymocyte subset
Function: Costimulatory signal for T and B cell activation, T cell development
Ligand Receptor: CD70
Interaction: TRAF2, TRAF5, CD27-binding protein (Siva)
Antigen References:

1. Hintzen RQ, et al. 1994. Immunol. Today 15:307.
2. Akiba H, et al. 1998. J. Biol. Chem. 273:13353.
3. Kobata T, et al. 1995. P. Natl. Acad. Sci. USA 92:11249.
4. Hendriks J, et al. 2000. Nat. Immunol. 1:433
5. Hintzen RQ, et al. 1994. Int. Immunol. 6:477
6. Camerini D, et al. 1991. J. Immunol. 147:3165


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
COMMERCIAL USE shall include:

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