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Safety Data Sheet (SDS) PE/Cy7 Anti-mouse IgM Antibody     Product Data Sheet (PDF)    
PE/Cy7 Anti-mouse IgM Antibody
2632565 25 µg $80.00       
2632570 100 µg $190.00       
Clone: RMM-1
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed

C57BL/6 splenocytes stained with CD19

C57BL/6 splenocytes stained with CD19 (6D5) APC and RMM-1 PE/Cy7

C57BL/6 splenocytes stained with CD19

C57BL/6 splenocytes stained with CD19 (6D5) APC and rat IgG2a PE/Cy7 isotype control



Description:

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.

Other Names: Immunoglobulin M
Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis

This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
COMMERCIAL USE shall include:

  • sale, lease, license or other transfer of the material or any material derived or produced from it;
  • sale, lease, license or other grant of rights to use this Material or any material derived or produced from it;
  • use of this material to perform services for a fee for third parties.

IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE RETURN THIS MATERIAL, UNOPENED TO Sony Biotechnology Inc., 2100 South Oak Street, Champaign, IL 61820, USA AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED.